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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 1401 - 1420 of 16276 results

  2. pHL-EF1a-SphcCas9-iP-A

    Plasmid
    #60599
    Purpose
    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon usage optimized for human usage
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    60599-plasmid-map-sequence-id-97109

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  6. pCW57-RFP-P2A-MCS (Neo)

    Plasmid
    #89182
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    89182-plasmid-map-sequence-id-176305

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  7. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    86612-plasmid-map-sequence-id-168849

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  11. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84241
    Purpose
    Expresses ABA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    84241-plasmid-map-sequence-id-160882

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  12. mRFP-wGBD

    Plasmid
    #26733
    Depositor
    William Bement
    Publication
    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)
    Use
    Reporter for cdc42
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    Cdc42 binding domain of N-WASP
    Available since
    April 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  18. CARMEN

    Plasmid
    #240464
    Purpose
    Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER
    Depositor
    Roland Malli
    Publication
    Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CARMEN
    Expression
    Mammalian
    Available since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240464-plasmid-map-sequence-id-482332

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Showing: 1401 - 1420 of 16276 results