We narrowed to 7,189 results for: cas9 plasmid

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pX330a dCas9-LSD1

Plasmid

#92362

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-LSD1

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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LentiV2-EF1α-nCas9/RT

Plasmid

#210188

Purpose

Expression of nCas9/RT-P2A-Puro

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

nCas9/RT-P2A-Puro

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-C9orf72

Plasmid

#83439

Purpose

Expresses Cas9 and a gRNA targeting C9orf72

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human C9orf72 (C9orf72 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpyCas9

Plasmid

#113034

Purpose

AAV vector for expression of SpyCas9 in mammalian cells

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-CI-SaCas9-W3SynpA

Plasmid

#231369

Purpose

minBG-driven SaCas9, with short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ-dpcoCas9-SunTag

Plasmid

#158410

Purpose

CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ150-Cas9(dpiRNA)

Plasmid

#107940

Purpose

eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

Cas9(dpiRNA)

Expression

Worm

Promoter

eft-3

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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