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pDONR221 NLRP1 delta787-843

Plasmid

#166821

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

_787-843

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Tags

Ubiquitin

Mutation

P1214R

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PIWIL2 ts2

Plasmid

#115870

Purpose

PIWIL2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PIWIL2 shRNA (PIWIL2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pPN161

Plasmid

#91580

Purpose

Express sgRNA targeting human CACNB2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CACNB2 (CACNB2 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN014

Plasmid

#91588

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN082

Plasmid

#91589

Purpose

Express sgRNA targeting human CUL3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CUL3 (CUL3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-wt

Plasmid

#67909

Purpose

Inducible expression of PIAL2 in E.coli

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAN1646

Plasmid

#127205

Purpose

pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2

Plasmid

#132775

Purpose

Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p27-mVenus reporter (PB)

Plasmid

#190731

Purpose

The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

mVenus
p27

Expression

Bacterial and Mammalian

Mutation

p27-F62A-F64A

Promoter

EF1α

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pIDK-HDAC1fl

Plasmid

#109158

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

Plasmid

#183790

Purpose

A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

Depositor

Jay Shendure

Article

Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

Inserts

P2A-eGFP-TargetBC-5xTAPE-1
pegRNA-InsertBC

Use

Synthetic Biology

Mutation

G>A in the gRNA scaffold- please see depositor…

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 WT

Plasmid

#231585

Purpose

ALPK1 WT gene under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDHODH-4

Plasmid

#186022

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDHODH-2

Plasmid

#186023

Purpose

knock out DHODH in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

Plasmid

#135955

Purpose

Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) N-terminal

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-hMAPT

Plasmid

#231583

Purpose

Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

human MAPT (MAPT Human)

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ChRmine-mScarlet

Plasmid

#137158

Purpose

Viral expression of ChRmine-p2a-mScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

ChRmine-p2a-mScarlet

Use

AAV

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

Plasmid

#166804

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S1213A

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-HDAC1 ts1

Plasmid

#115852

Purpose

HDAC1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HDAC1 shRNA (HDAC1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-HDAC1 ts3

Plasmid

#115849

Purpose

HDAC1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HDAC1 shRNA (HDAC1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-HDAC1 ts2

Plasmid

#115848

Purpose

HDAC1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HDAC1 shRNA (HDAC1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

Plasmid

#107700

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3-P2A-CheRiff

Use

AAV

Expression

Mammalian

Promoter

human synapsin I

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

Plasmid

#166794

Purpose

Mammalian cell expression vector for Flag-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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YA1532: pAAV_ CAG-FLEX-paQuasAr3

Plasmid

#107702

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SETDB1 ts1

Plasmid

#115859

Purpose

SETDB1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SETDB1 shRNA (SETDB1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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