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Showing: 2841 - 2880 of 25948 results

  6. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Insert
    InPAkt
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  12. psiCHECK2-miR-34 MT

    Plasmid
    #78259
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated miR-34 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CCGT to GG…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78259-plasmid-map-sequence-id-149444

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  19. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid
    #136646
    Purpose
    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC
    Depositor
    Karl Matter, Maria Balda
    Article
    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.
    Insert
    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    Use
    Tags
    HA (MQDLPGNDNSTAG)
    Expression
    Mammalian
    Mutation
    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136646-plasmid-map-sequence-id-271745

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  34. pET28a-HsSENP5

    Plasmid
    #71468
    Purpose
    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2841 - 2880 of 25948 results