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pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235321

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235313

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235320

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235319

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235314

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235322

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235324

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx6-mNgn2x3HA-P2A-mRuby2

Plasmid

#233168

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFwx1-Isl1-P2A-mRuby2

Plasmid

#233170

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx1-Isl1-P2A-mRuby2

Plasmid

#233171

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx2-Isl1-P2A-mRuby2

Plasmid

#233172

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx3-Isl1-P2A-mRuby2

Plasmid

#233173

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx6-Isl1-P2A-mRuby2

Plasmid

#233174

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFwx1-mLhx3-P2A-mRuby2

Plasmid

#233176

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx1-mLhx3-P2A-mRuby2

Plasmid

#233177

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx2-mLhx3-P2A-mRuby2

Plasmid

#233178

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx3-mLhx3-P2A-mRuby2

Plasmid

#233179

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx6-mLhx3-P2A-mRuby2

Plasmid

#233180

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Synthetic, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFwx1-mNgn2x3HA-P2A-mRuby2

Plasmid

#233164

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx1-mNgn2x3HA-P2A-mRuby2

Plasmid

#233165

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx2-mNgn2x3HA-P2A-mRuby2

Plasmid

#233166

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-uORFx3-mNgn2x3HA-P2A-mRuby2

Plasmid

#233167

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter with upstream open reading frame

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hTln1-R1R2

Plasmid

#191440

Purpose

Expresses the human TLN1 R1R2 domains in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS10-riboE-dddAI

Plasmid

#186561

Purpose

Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

pSpac(hy)

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-HaloSFPQY527A

Plasmid

#166949

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
UbC-Halo-SFPQY527A

Plasmid

#166947

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

UbC

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHSU74.2-2.2

Plasmid

#115543

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7

Expression

Yeast

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHKU16.1-2.2

Plasmid

#115529

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHKU20.1-2.2

Plasmid

#115531

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHSU68.2-2.2

Plasmid

#110681

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHKU27.2-2.2

Plasmid

#110662

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHSU75.2-2.2

Plasmid

#110691

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHKU33.2-2.2

Plasmid

#110667

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FNE2-A72

Plasmid

#44608

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2-A72 (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

THR-72 converted to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a I18A

Plasmid

#40043

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Isoleucine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

Plasmid

#106171

Purpose

cre mediated red to green shift

Depositor

Ewa Snaar-Jagalska

Article

Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

Inserts

dsRED-express2
eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet1-dCas9

Plasmid

#136650

Purpose

Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

Tet1 (Tet1 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Tet1-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-KLC1

Plasmid

#166964

Purpose

Expresses Myc-tagged KLC1 protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-GFP-SFPQ-IRES-mCherry

Plasmid

#166950

Purpose

Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Use

Lentiviral

Tags

GFP and mCherry

Promoter

eF1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-G418-YR

Plasmid

#61767

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

nptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

Plasmid

#166951

Purpose

Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

GFP and mCherry

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
HA-KIF5A

Plasmid

#166958

Purpose

Expresses HA-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1596 - pAAV SYN1 HA-hM3D(Gq)

Plasmid

#121539

Purpose

An adeno-associated viral vector expressing HA-tagged stimulatory DREADD (hM3D(Gq)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV Retrograde, AAV2, and AAV5

Insert

HA-tagged hM3D(Gq) (CHRM3 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

SYN1

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-KIF5A

Plasmid

#166954

Purpose

Expresses FLAG-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI-FLAG

Plasmid

#186560

Purpose

Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Tags

FLAG

Expression

Bacterial

Promoter

tac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-HA-2xMCP-Ezh2

Plasmid

#126590

Purpose

Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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