We narrowed to 74,867 results for: Rest

1. pMEXneo-TrkC

   Plasmid

   #190202

   Purpose

   To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Use

   Eucaryotic

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney mouse sarcoma virus LTR

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-mR2-ANLN_sgRNA

   Plasmid

   #183876

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2144

   Plasmid

   #104357

   Purpose

   AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

   Insert

   ssAAV-smCBA-EmGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   smCBA

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-HypaCas9-mR2-RHOA_sgRNA

   Plasmid

   #183877

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA sgRNA spacer (RHOA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. nes714tk/lacZ

   Plasmid

   #47614

   Purpose

   used to create transgenic mice expressing LacZ reporter with Nestin enhancer

   Depositor

   Urban Lendahl

   Article

   Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

   Insert

   nestin 2nd intron fragment (NES Human)
   

   Use

   Enhancer reporter

   Tags

   HSV tk promoter and lacZ

   Expression

   Mutation

   714 bp from 3' end of 2nd intron

   Promoter

   HSV tk promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

6. nes1852tk/lacZ

   Plasmid

   #47613

   Purpose

   used to create transgenic mice expressing LacZ reporter with Nestin enhancer

   Depositor

   Urban Lendahl

   Article

   Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

   Insert

   nestin 2nd intron (NES Human)
   

   Use

   Enhancer reporter

   Tags

   HSV tk promoter and lacZ

   Expression

   Mutation

   2nd intron only

   Promoter

   HSV tk promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. 1068 pGL3 FasL promoter (no SV40 prom)

   Plasmid

   #9029

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Removed the SV40 promoter from pGL3

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. ND4-FusXTBE-Left TALE

   Plasmid

   #196496

   Purpose

   Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ND4-FusXTBE-Right TALE

   Plasmid

   #196497

   Purpose

   Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

   Insert

   COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCDH_CMV_HMGA_linker_mKate2

    Plasmid

    #167335

    Purpose

    Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) as well a…

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB6 (human) FLAG pCMV

    Plasmid

    #8815

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. HOXB6 (human) EGFP-fusion

    Plasmid

    #8535

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains eGFP fused to N-terminal of HOXB6 at AA…

    Promoter

    CMV

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid

    #229509

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    RbcX2
    BSD2
    RbcX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 2xSTREP Cyclin F(LP35/36AA)

    Plasmid

    #236467

    Purpose

    transient overexpression of Cyclin F in mammalian cells

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

    Insert

    Cyclin F (CCNF Human)
    

    Use

    Tags

    2xSTREP

    Expression

    Mammalian

    Mutation

    (LP35/36AA) first two amino acids of the F-box do…

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-KDEL_SBP-EGFP-ColX

    Plasmid

    #222305

    Purpose

    Synchronize the trafficking of CollagenX from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide of COL10A1 removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-KDEL_SBP-EGFP-gp135

    Plasmid

    #222306

    Purpose

    Synchronize the trafficking of gp135/podocalyxin from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCRISPRv2_sgALDH7A1

    Plasmid

    #218273

    Purpose

    CRISPR KO plasmid for ALDH7A1 in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas_4(I-U)/1_2_VB

    Plasmid

    #186445

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain swapped with Cas4 domain from G. sulf…

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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