We narrowed to 75,846 results for: Rest

1. pCS2_IRES2_CyOFP1_KCNQ2 var4

   Plasmid

   #173155

   Purpose

   For mammalian expression of KCNQ2 and creating variants

   Depositor

   Al George

   Article

   Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

   Insert

   KCNQ2 (KCNQ2 Human)
   

   Use

   Tags

   IRES2-CyOFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Simian CMV IE94

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK+ chickBMPR-1B CA

   Plasmid

   #49528

   Purpose

   cloning vector for constitutively active chick BMP receptor 1B

   Depositor

   Peter ten Dijke, Lee Niswander

   Article

   Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

   Insert

   BMPR-1B CA (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   constitutively active mutation (Q203D); also P2A,…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. K785R Smarca4-sfGFP

   Plasmid

   #107057

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

   Depositor

   Courtney Hodges, Jerry Crabtree

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   Super-folder GFP (sfGFP)

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. K785R Smarca4-V5 IRES-puro

   Plasmid

   #107059

   Purpose

   Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

   Depositor

   Courtney Hodges, Jerry Crabtree

   Article

   Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-puroR and V5

   Expression

   Mammalian

   Mutation

   K785R

   Promoter

   CAG

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-Puro-IKBalpha-wt

   Plasmid

   #15290

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pCIneoMyc UNC119Aa

   Plasmid

   #128331

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119A isoform a

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119 (UNC119 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mGas8-PA

   Plasmid

   #176468

   Purpose

   Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   growth arrest specific 8 (Gas8 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneoMyc UNC119Ab

    Plasmid

    #128332

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119A isoform b

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119 (UNC119 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pX459-HypaCas9-RHOA_sgRNA

    Plasmid

    #183878

    Purpose

    pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. aP2-GFPtpz

    Plasmid

    #114211

    Purpose

    aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

    Depositor

    David Rowe

    Article

    Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

    Insert

    aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Mouse, Synthetic, Aequorea victoria)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    aP2/Fabp4

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-ACTB_sgRNA

    Plasmid

    #183885

    Purpose

    pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1 KanR foundation lox casette

    Plasmid

    #132388

    Purpose

    Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    G418 resistance
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    promoterless

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human_TFAM_dHMGA_pET28

    Plasmid

    #167329

    Purpose

    Expresses TFAM dHMGA mutant in bacterial cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Truncation mutant containing linker, HMGB, and C-…

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEXneo-TrkC

    Plasmid

    #190202

    Purpose

    To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Use

    Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney mouse sarcoma virus LTR

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. nes714tk/lacZ

    Plasmid

    #47614

    Purpose

    used to create transgenic mice expressing LacZ reporter with Nestin enhancer

    Depositor

    Urban Lendahl

    Article

    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

    Insert

    nestin 2nd intron fragment (NES Human)
    

    Use

    Enhancer reporter

    Tags

    HSV tk promoter and lacZ

    Expression

    Mutation

    714 bp from 3' end of 2nd intron

    Promoter

    HSV tk promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only