We narrowed to 162,289 results for: addgene

1. pZNF462.1.0-gDNA

   Plasmid

   #112476

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462

   Depositor

   Kevin White

   Insert

   ZNF462 (ZNF462 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBPL1.1.0-gDNA

   Plasmid

   #112412

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

   Depositor

   Kevin White

   Insert

   TBPL1 (TBPL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMAD3.1.0-gDNA

   Plasmid

   #112407

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

   Depositor

   Kevin White

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. WNK2 (WNK2A-c003)

   Plasmid

   #110250

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   WNK2 (WNK2 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5JJW (USP15_SART3)

   Plasmid

   #101880

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15_SART3 (USP15, SART3 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 2MJ8

   Plasmid

   #61956

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CDYL2 (CDYL2 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. TDRD7 (3RCO)

   Plasmid

   #32051

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD7 (TDRD7 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 21, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. GIMAP2

   Plasmid

   #28166

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   GTPase IMAP family member 2 (GIMAP2 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   AIG1 domain only

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVTRE3_Clover

    Plasmid

    #135179

    Purpose

    AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-XL-AncBE4max

    Plasmid

    #136254

    Purpose

    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    AncBE4max_GFP
    

    Use

    CRISPR; Piggybac dna transposon

    Tags

    Expression

    Mammalian

    Mutation

    The AncBE4max_GFP insert was PCR amplified from a…

    Promoter

    TRE3G

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK Zeo p53DD

    Plasmid

    #136534

    Purpose

    Lentiviral expression vector for an inducible p53DD

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    This construct was created from the pBabe p53DD (…

    Promoter

    Available since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #135012

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCriprV2-sgRNA-PER1-#1

    Plasmid

    #189987

    Purpose

    Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Period1 (PER1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-SLC35B2-FLAG

    Plasmid

    #154863

    Purpose

    Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ctcctggtgcagtacttc => ttattagtccaatatttt

    Promoter

    CMV

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN_TTmiRC2_p53DD

    Plasmid

    #136520

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    This construct was created by PCR from the pBabe …

    Promoter

    Available since

    Oct. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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