We narrowed to 159,671 results for: addgene

1. pPD95.77-wVenus

   Plasmid

   #37465

   Depositor

   Hitoshi Sawa

   Article

   Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMBPcyto-TEV-BseRI

   Plasmid

   #157739

   Purpose

   empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   His10 + MBP (cytoplasmic expression; no signal se…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBES2

   Plasmid

   #83800

   Purpose

   Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6D1T (MBD1)

   Plasmid

   #119966

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD1 (MBD1 Human)
   

   Use

   Tags

   GSTtag, 6xHIS and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pUPD2:35S-TMV-Rep-MP

   Plasmid

   #106217

   Purpose

   Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

   Depositor

   Tomáš Moravec

   Insert

   Infectious clone of Tobacco mosaic virus, 5' part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6WNX

   Plasmid

   #162270

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   FBXW11 (FBXW11 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. BAZ2B

   Plasmid

   #73229

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BAZ2B (BAZ2B Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00439:Bromodomain 1871-1955

   Promoter

   T7

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKG204

   Plasmid

   #63078

   Purpose

   Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   HP1 alpha (CBX5 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Mutation

   V151I mutation compared to GenBank reference sequ…

   Promoter

   Available since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVD621

   Plasmid

   #66162

   Purpose

   to make a ctxB probe

   Depositor

   James Kaper

   Article

   Michalski et al Infect Immun. 1993 Oct;61(10):4462-8.

   Insert

   ctxB
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

10. WDR39

    Plasmid

    #25308

    Purpose

    Expresses His-tagged WDR39 (CIAO1) in bacteria

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR39_1 (CIAO1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pKG326

    Plasmid

    #69753

    Purpose

    Retroviral expression of CENP-T aa 1-531

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-531 (CENPT Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    E.coli optimized sequence; A216T and G459R mutati…

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. 6XKC

    Plasmid

    #162271

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    FEM1C (FEM1C Human)
    

    Use

    Tags

    6xHis, GST and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    GST-F:tgaagctatcccacaaattg

    Available since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTBS 5Y YIC

    Plasmid

    #18786

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    TRAP binding site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PREX1

    Plasmid

    #32041

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PREX1 (PREX1 )
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    first PDZ domain only (amino acids Residues 607-7…

    Promoter

    Available since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. KCTD10

    Plasmid

    #73253

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KCTD10A (KCTD10 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD95.79-wVenus

    Plasmid

    #37466

    Depositor

    Hitoshi Sawa

    Article

    Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINDUCER20-hSRF-3xHA (#757)

    Plasmid

    #78344

    Purpose

    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

18. MLH1

    Plasmid

    #32859

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1 (MLH1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    contains amino acids 486-751

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pFOXA3.1.0-gDNA

    Plasmid

    #112417

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3

    Depositor

    Kevin White

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CYP11A1 (3N9Z/3NA0)

    Plasmid

    #28118

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP11A1:HPC071-D08:C40435 (CYP11A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits