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We narrowed to 54,060 results for: tra

Showing: 1421 - 1440 of 54060 results

  1. AAV-short_BrokenHeart (no BC)

    Plasmid
    #86950
    Purpose
    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
    Depositor
    Joseph Dougherty, Rob Mitra
    Article
    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    Brokenheart construct
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    86950-plasmid-map-sequence-id-172437

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  2. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  4. p35S_EVDdi

    Plasmid
    #167120
    Purpose
    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the intron under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE (EVD) (AT5G17125 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Deleted intron from full length element
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167120-plasmid-map-sequence-id-331755

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  5. pBGE3.0

    Plasmid
    #165988
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165988-plasmid-map-sequence-id-378724

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  6. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

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  7. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid
    #126473
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126473-plasmid-map-sequence-id-246528

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  8. pAGE1.0

    Plasmid
    #165983
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165983-plasmid-map-sequence-id-378731

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  9. pAGE3.0

    Plasmid
    #165985
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165985-plasmid-map-sequence-id-375864

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  10. p35S_EVDm1

    Plasmid
    #167121
    Purpose
    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE (EVD) (AT5G17125 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    mutated four nucleotides at the snoRNA U1 binding…
    Promoter
    CaMV 35S
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167121-plasmid-map-sequence-id-333657

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  11. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Use
    Tags
    GUS and mGFP5
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167123-plasmid-map-sequence-id-331746

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  12. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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  13. pHLsec-dasTMPRSS2

    Plasmid
    #207850
    Purpose
    Expresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cells
    Depositor
    Ingrid De Meester
    Article
    Verhulst et al J Extracell Vesicles. 2025 Mar;14(3):e70
    Insert
    transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Tags
    AviTag, 8xHIS tag
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-108, S251D R252D Q253D S254D…
    Promoter
    CAG
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    207850-plasmid-map-sequence-id-419957

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  14. pBW001

    Plasmid
    #131529
    Purpose
    Vector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactions
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mu-BsaI-KanR transposon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    131529-plasmid-map-sequence-id-263309

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  15. pCAG-EnvARGCD

    Plasmid
    #100800
    Purpose
    express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain
    Depositor
    Ian Wickersham
    Insert
    fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    100800-plasmid-map-sequence-id-189516

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  17. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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Showing: 1421 - 1440 of 54060 results