161,296 results

1. pAAV-CAG-GFP (AAV2 trial size)

   Viral Prep

   #37825-AAV2.T

   Purpose

   Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3 HA C9ORF72 op

   Plasmid

   #74156

   Purpose

   Express HA tagged optimized C9ORF72 in mammalian cells

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   C9ORF72 (C9orf72 Human, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   optimized sequence

   Promoter

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

   Viral Prep

   #137141-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pU6-Lb-crRNA

   Plasmid

   #78957

   Purpose

   Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-His-TEV-NQO1

   Plasmid

   #119058

   Purpose

   For expression in E.coli and affinity purification of N-terminally His tagged NQO1

   Depositor

   Yosef Shaul

   Article

   Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

   Insert

   NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
   

   Use

   Tags

   6His, TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV Retrograde)

   Viral Prep

   #154868-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ulrik Gether

   Promoter

   Human Synapsin-1

   Tags

   mCherry (Flp-dependent)

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-TRE-GFP-Blast

   Plasmid

   #135673

   Purpose

   Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep

    #100846-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 (#100846). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    CAG

    Tags

    None

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only