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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-raptor

Plasmid

#73385

Purpose

Expresses YFP and HA tagged Human raptor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
Mut1-RS

Plasmid

#182881

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut1-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-3XMyc-mTrim25

Plasmid

#51394

Depositor

Narry Kim

Article

Kwon et al Nat Struct Mol Biol. 2013 Sep;20(9):1122

Insert

Trim25 (Trim25 Mouse)

Use

Lentiviral

Tags

3XMyc

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pKH142_EGFP-YIPF3 WT

Plasmid

#214974

Purpose

GFP tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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