We narrowed to 7,945 results for: Lif;
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pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterSNR52Available SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
raACR-pmCerulean3-N1
Plasmid#204959PurposeExpression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cellsDepositorInsertraACR
TagsmCerulean3ExpressionMammalianPromoterCMV (+enhancer)Available SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjBCCIP
Plasmid#122278PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjBCCIP 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha
Plasmid#179713PurposeLentiviral expression of mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMito-dCherry-FRB
Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
ExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCR6-eLOV-TEVcs-tTA
Plasmid#173116PurposeExpresses CCR6 receiver construct in neuronsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVPromoterSynapsinAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68
Plasmid#162650PurposeExpresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Tyr
Plasmid#170293PurposeA knock-out vector for the mouse tyrosinase.DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
TagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
CALNL-RARa-VP16
Plasmid#185560PurposeCre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alphaDepositorAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-Nuf2
Plasmid#80760PurposeExpress Nuf2 N-terminally labeled with mTurquoise2DepositorAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PM-CaNARi
Plasmid#64850PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertLyn-CaNARi
TagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCS2-Opto-Alk8
Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)
Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-PP-GFP
Plasmid#135390PurposeExpresses OSCA1.2 in mammalian cellsDepositorInsertOSCA1.2 (AT4G22120 Mustard Weed)
TagsEGFP tag connected to the gene via a precision pr…ExpressionMammalianPromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6b-T
Plasmid#117210PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6b promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only
