We narrowed to 3,143 results for: N-EGFP

1. TMEM16F-M522K-peGFP-N1

   Plasmid

   #228579

   Purpose

   Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M522K plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TMEM16F-T526K-peGFP-N1

   Plasmid

   #228580

   Purpose

   Expresses mouse TMEM16F-T526K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T526K plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EHD2-T72A-mEGFP

   Plasmid

   #45934

   Purpose

   dominant-negative EHD2 mutant

   Depositor

   Ari Helenius

   Article

   Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

   Insert

   Eps-15 homology domain-containing protein2 (EHD2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   T 72 changed to A (T72A)

   Promoter

   CMV

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsDCX-EGFP-pHAT

   Plasmid

   #83918

   Purpose

   human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

   Depositor

   Gary Brouhard

   Article

   Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

   Insert

   Doublecortin isoform b (DCX NM_178152.2, Human)
   

   Use

   Tags

   6-His and EGFP+Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE ENTR eGFP L5-L2

   Plasmid

   #62183

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   eGFP
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TMEM16F-I521A-peGFP-N1

   Plasmid

   #228577

   Purpose

   Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I521A plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TMEM16F-I521E-peGFP-N1

   Plasmid

   #228578

   Purpose

   Expresses mouse TMEM16F-I521E-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I521E plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmApple-EGFP-HRas

   Plasmid

   #133854

   Purpose

   Mammalian expression of HRas N-terminally tagged with mApple and EGFP

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   HRas (HRAS Human)
   

   Use

   Tags

   EGFP and mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)

   Plasmid

   #154470

   Purpose

   cDNA of NPM-ALK in the MSE backbone

   Depositor

   Cornelius Miething

   Article

   Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

   Insert

   NPM-ALK
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid

    #195854

    Purpose

    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    6xHis-AviTag-ALFAtag-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT-TO-EGFP-UPF1-R615A

    Plasmid

    #136001

    Purpose

    DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R615A

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5 FRT-TO-EGFP-UPF1-DEAA

    Plasmid

    #136002

    Purpose

    DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DE647AA

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor

    Plasmid

    #211867

    Purpose

    CENP-T(1-242) transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T(1-242) (CENPT Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid

    #223087

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid

    #223079

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid

    #223102

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid

    #223103

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid

    #223104

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)

    Plasmid

    #223105

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge