We narrowed to 2,364 results for: PUC19

1. pJAID6JY

   Plasmid

   #165049

   Purpose

   Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAID8Z105

   Plasmid

   #165057

   Purpose

   Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAID9L105

   Plasmid

   #165055

   Purpose

   Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAID9L

   Plasmid

   #165052

   Purpose

   Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAID5Z

   Plasmid

   #165047

   Purpose

   Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDiySpike3

   Plasmid

   #136950

   Purpose

   Plasmid for the In Vitro transcription of DiySpike3

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike3
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDiySpike1d1

   Plasmid

   #136951

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d1

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d1
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDiySpike1d2

   Plasmid

   #136952

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d2

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d2
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDiySpike1d3

   Plasmid

   #136953

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d3

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d3
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDiySpike1d4

    Plasmid

    #136954

    Purpose

    Plasmid for the In Vitro transcription of DiySpike1d4

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike1d4
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDiySpike2d1

    Plasmid

    #136955

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2d1

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2d1
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDiySpike2d2

    Plasmid

    #136956

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2d2

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2d2
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDiySpike2d3

    Plasmid

    #136957

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2d3

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2d3
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDiySpike2d4

    Plasmid

    #136958

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2d4

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2d4
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP553‐1

    Plasmid

    #87240

    Purpose

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
    

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas28

    Plasmid

    #82385

    Purpose

    sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23108

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas27

    Plasmid

    #82384

    Purpose

    sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23117

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAD06 K. lactis telomerase RNA hairpin

    Plasmid

    #61895

    Purpose

    Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin (nt 229-273)
    

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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