-
Plasmid#187223PurposeExpresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-RFP
Plasmid#65448PurposeExpression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectorsDepositorInsertSV40-RFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2-T2A-HO1
Plasmid#179485PurposeExpresses miRFP2 and HO1in worm cellsDepositorInsertwmIRFP2-T2A-HO1
UseTagsExpressionMammalianMutationNOPromotertag-168 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-TAF15N-miRFP670-Cry2WT
Plasmid#122440PurposeExpresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-iRFP670
Plasmid#110694PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670DepositorInsertCPSF6-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationTruncation of CPSF6 isoform at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-TagBFP
Plasmid#117768PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-CaURA3
Plasmid#44882PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP-DN-FOXO1-HA neo
Plasmid#45813DepositorInsertFOXO1 (FOXO1 Human)
UseRetroviralTagsHA and RFPExpressionMammalianMutationaa1-255PromoterAvailable sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
keratin 8 (krt8)-H2B-RFP
Plasmid#105955PurposetransgenesisDepositorInsertkrt8 (KRT8 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-sTagRFP-TUBA1B
Plasmid#207768PurposeDonor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2KXIGdeltaIn-MCS-birA-tagRFP
Plasmid#58378Purposetol2 transgenesis for biotin based TRAP - BirA ligaseDepositorInsertbirA tagRFP
UseTagstagRFPExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRTS-GBP-dCas9-mRFP
Plasmid#102934PurposeTetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)DepositorInsertdCas9
UseCRISPRTagsGBP (GFP binding protein and mRFPExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-cmlc2-mRFP
Plasmid#117775PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1wt-IRES-RFP
Plasmid#33334DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-eGFP
Plasmid#117770PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-mRFP-pcDNA3.1-
Plasmid#52516Purposeexpresses mRFP in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertmRFP
UseTagsRGS-6xHisExpressionMammalianMutationengineered variant of tetrameric red fluorescent …PromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 TagRFP-T LGALS3 R186S
Plasmid#202428PurposeExpression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsTagRFPExpressionMutationR186SPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorUseTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-mRFP
Plasmid#117784PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only