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Plasmid#124399PurposeFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624) and L897A/…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-GFP 1-10 tetra Cys
Plasmid#78589PurposeExpression flag-GFP 1-10 tetra Cys in mammalian cellsDepositorInsertflag-GFP 1-10 tetra Cys
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GST
Plasmid#41026DepositorInserttetO7-Pcyc1-GST
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLtetO1-deGFP-T500
Plasmid#45392DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterPL-tetO1Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA
Plasmid#41024DepositorInserttetO7-Pcyc1-3xHA
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorInserttetracycline-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo CXCR4-sh3
Plasmid#163743PurposeLentivirus for inducible knockdown of CXCR4 (human)DepositorInsertCXCR4 (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo CXCR4-sh1
Plasmid#163741PurposeLentivirus for inducible knockdown of CXCR4 (human)DepositorInsertCXCR4 (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO-neo CXCR4-sh2
Plasmid#163742PurposeLentivirus for inducible knockdown of CXCR4 (human)DepositorInsertCXCR4 (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on-puromycin-shTRX1-259
Plasmid#110920PurposeTo knockdown Thioredoxin-1 following doxycycline additionDepositorInsertTRX1 (TXN Human)
UseLentiviralTagsExpressionMutationPromoterunknownAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-Incenp-EGFP
Plasmid#108483Purposeinducible expression of vsv-INCENP wt-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA
Plasmid#121806PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETaDepositorInsertHA epitope tag-polyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG
Plasmid#129758PurposeDox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianMutationchanged Glutamic acid 36 to Alanine and Leucine 3…PromoterTRE3Gs and Tet inducible promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1d834-1363-Poly(A)
Plasmid#65560PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationdel(834-1363)PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pES005 (pTet-qacR wt/pQacA-deGFP)
Plasmid#69034Purposereporter with wild-type qacR on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationPromoterpQacA and pTetAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1d834-1053-Poly(A)
Plasmid#65561PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationdel(834-1053)PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-UCP1-Flag
Plasmid#234983PurposeExpresses UCP1 with C-terminal Flag tag in human cell linesDepositorInsertUCP1 (Ucp1 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only