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We narrowed to 6,244 results for: cas9 expression plasmid

Showing: 1441 - 1460 of 6244 results

  1. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  2. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  3. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72250-plasmid-map-sequence-id-131871

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  5. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  6. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  7. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  8. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  9. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  11. pXPR_047

    Plasmid
    #107145
    Purpose
    measures Cas9 activity
    Depositor
    David Root
    Insert
    GFP + sgRNA for GFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107145-plasmid-map-sequence-id-205295

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  12. pORFE0001

    Plasmid
    #112070
    Purpose
    AtCas9 module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112070-plasmid-map-sequence-id-217624

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  16. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Matthias Heinemann
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166111-plasmid-map-sequence-id-330321

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  17. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

    Viral Prep
    #112677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep
    #112677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

    Viral Prep
    #112677-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral Prep
    #112677-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1460 of 6244 results