We narrowed to 13,592 results for: crispr cas9

1. pCMV-Cas9-NRRH

   Plasmid

   #136924

   Purpose

   Expresses Cas9-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRRH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3-SauriCas9-BlastR

   Plasmid

   #220967

   Purpose

   Expresses human codon-optimized SauriCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-tre-dCas9-VPR

   Plasmid

   #227506

   Purpose

   piggyBac integration and dox-inducible expression of VPR-dCas9

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   VPR-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Sga-Cas9

   Plasmid

   #227005

   Purpose

   Mammalian expression of Nuclease active S. gallolyticus Cas9

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Sin-Cas9

   Plasmid

   #227006

   Purpose

   Mammalian expression of Nuclease active S. iniae Cas9

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Spa-Cas9

   Plasmid

   #227007

   Purpose

   Mammalian expression of Nuclease active S. parasanguinis Cas9

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Sub-Cas9

   Plasmid

   #227008

   Purpose

   Mammalian expression of Nuclease active S. uberis Cas9

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3-Nm2Cas9-BlastR

    Plasmid

    #220980

    Purpose

    Expresses human codon-optimized Nm2Cas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    Nm2Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.3-CjCas9-BlastR

    Plasmid

    #220979

    Purpose

    Expresses human codon-optimized CjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    CjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti6.3-St1Cas9-BlastR

    Plasmid

    #220976

    Purpose

    Expresses human codon-optimized St1Cas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    St1Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.3-SlugCas9-HF-BlastR

    Plasmid

    #220973

    Purpose

    Expresses human codon-optimized SlugCas9-HF

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SlugCas9-HF
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.3-Sa-SlugCas9-BlastR

    Plasmid

    #220968

    Purpose

    Expresses human codon-optimized Sa-SlugCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    Sa-SlugCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Native promoter dCas9

    Plasmid

    #113656

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    Native promoter and dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    S. pyogenes Cas9 native promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid

    #139992

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid

    #139990

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VQR=D1135V/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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