We narrowed to 2,218 results for: dcas9

1. JC101: pMVP/Ad/PK-DEST

   Plasmid

   #121845

   Purpose

   pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

   Plasmid

   #84256

   Purpose

   Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgCXCR4-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

   Plasmid

   #84255

   Purpose

   Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgCXCR4-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. IO001: pMVP (R4-R3) eGFP

   Plasmid

   #121730

   Purpose

   pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eGFP
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. JN607: pMVP/SB-DEST

   Plasmid

   #121860

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174119

   Purpose

   Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KK402: pMVP/Zeo-DEST

   Plasmid

   #121859

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. IO201: pMVP (R4-R3) mCherry

   Plasmid

   #121732

   Purpose

   pMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

   Plasmid

   #192681

   Purpose

   Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. IG401: pMVP (L1-R5) RIP

    Plasmid

    #121694

    Purpose

    pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    RIP
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KJ801: pMVP/Hygro-DEST

    Plasmid

    #121858

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JL302: pMVP/Puro-DEST

    Plasmid

    #121856

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMP8921

    Plasmid

    #220903

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP8923

    Plasmid

    #220904

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP8932

    Plasmid

    #220906

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. gRNA-hIRF-1 #12/pSIR-hCD2

    Plasmid

    #135392

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    gRNA_hIRF1 promoter #12
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GB2890

    Plasmid

    #193133

    Purpose

    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1dG2b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GB1839

    Plasmid

    #193103

    Purpose

    GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP gRNA2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK411.RR1

    Plasmid

    #192644

    Purpose

    Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    BBa_J23119-RR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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