We narrowed to 2,362 results for: dcas9

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pMOD8A-iRGR-r11

Plasmid

#74369

Purpose

This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

RGR-r11

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

AHD1

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pEF:iCas9

Plasmid

#138267

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in human.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Mammalian

Mutation

Cas9-D10A, H840A

Promoter

Ef1a

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_Puro

Plasmid

#167887

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_EYFP

Plasmid

#167882

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R0

Plasmid

#101803

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCas2F

Plasmid

#82377

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EYFP

Plasmid

#167866

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCas2D

Plasmid

#82375

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R20

Plasmid

#101818

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G20-R0

Plasmid

#101807

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJHCi

Plasmid

#251729

Purpose

broad-host-range plasmid for CRISPR interference; inducible dCas9; sgRNA expression from methanotroph constitutive promoter

Depositor

Calvin Henard

Article

Henard et al Sci Adv. 2026 Apr 24;12(17):eaed4234. do

Insert

dCas9

Use

CRISPR

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pSC-pGAL10-CRISPRa

Plasmid

#240210

Purpose

Yeast vector for integration of pGAL10-dCas9 and pGAL10-MCP-VPR at LEU2 locus

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

MCP-VPR
dCas9

Expression

Yeast

Promoter

pGAL10

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDA010.188

Plasmid

#206792

Purpose

J23113.dCas9

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

dCas9

Promoter

J23113

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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GB2880

Plasmid

#193117

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.6

Use

Synthetic Biology

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB3277

Plasmid

#193131

Purpose

A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1abc.4

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB2888

Plasmid

#193126

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.4

Use

Synthetic Biology

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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GB2886

Plasmid

#193124

Purpose

A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1ab.3

Use

Synthetic Biology

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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GB2883

Plasmid

#193120

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.4

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB2882

Plasmid

#193119

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.3

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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