We narrowed to 2,134 results for: dcas9

1. pMT1-24X-NOG(CmYLCV)

   Plasmid

   #202014

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LC301: pMVP/PB/Zeo-DEST

   Plasmid

   #121877

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IY601: pMVP/Lenti/Blast-DEST

   Plasmid

   #121849

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT1-24X-NOG(AtUBI10)

   Plasmid

   #202016

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Inserts

   dCas9-24XGP41
   NbGP41P-sfGFP-VP64-GB1
   

   Use

   Tags

   GB1 and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPReporter

   Plasmid

   #65007

   Purpose

   E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. LC001: pMVP/PB/Hygro-DEST

   Plasmid

   #121876

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. IF101: pMVP (L1-L4) EF1a promoter

   Plasmid

   #121687

   Purpose

   pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   EF1a promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HB401: pMVP (L1-L4) CMV promoter

    Plasmid

    #121686

    Purpose

    pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

    Plasmid

    #119059

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1b minimal promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. (401) pAAV CMV-ZsGreen U6-gRNA

    Plasmid

    #163021

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. JH401: pMVP/Ad/RGD-PK-DEST

    Plasmid

    #121846

    Purpose

    pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. IG301: pMVP (L1-R5) CMV promoter

    Plasmid

    #121692

    Purpose

    pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. JEH127

    Plasmid

    #179299

    Purpose

    Expresses dCas9 fused to VPR

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KK001: pMVP/SB/Hygro-DEST

    Plasmid

    #121867

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge