We narrowed to 16,609 results for: puromycin

1. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

   Plasmid

   #73707

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   T231A/S232A

   Promoter

   CMV/tet

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

   Plasmid

   #73702

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   448-AAAA-451

   Promoter

   CMV/tet

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pPyCAG-Nanog(K137A)-IP

   Plasmid

   #62996

   Purpose

   Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog K137A Mutant (Nanog Mouse)
   

   Expression

   Mammalian

   Mutation

   Changed Lysine 137 (138 AA in Mouse Protein) to A…

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPyCAG-Nanog(Y119A)-IP

   Plasmid

   #62991

   Purpose

   Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Y119A Mutant (Nanog Mouse)
   

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 119 (120 AA in Mouse Protein) to…

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYJ41-AAVS1-XLone-Mafa

   Plasmid

   #131084

   Purpose

   Donor plasmid of targeted inducible-Mafa knockin

   Depositor

   Xiaojun Lian

   Insert

   MAFA (MAFA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMbd3-GGSG-AID-3xF_PP

   Plasmid

   #235507

   Purpose

   Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed Pgk-Puro

   Depositor

   Brian Hendrich

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID, 3xFLAG

   Mutation

   None

   Promoter

   PGK

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti puro HA-STX3

   Plasmid

   #227590

   Purpose

   Lentiviral plasmid expressing human STX3 protein

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   STX3 (STX3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX ALIX-GFP S718/721A

   Plasmid

   #138583

   Purpose

   Expresses fluorescent ALIX S718/721A in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Mutation

   changed serines 718 and 721 to alanines

   Promoter

   TREtight

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1-GFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132622

   Purpose

   Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-puro-hCASP8

    Plasmid

    #185378

    Purpose

    For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)

    Depositor

    Ayaz Najafov

    Insert

    CASP8 (CASP8 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-puro-hARAF-1

    Plasmid

    #185367

    Purpose

    For mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAF

    Depositor

    Ayaz Najafov

    Insert

    ARAF (ARAF Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-puro-mAK1-shRNA-1

    Plasmid

    #185364

    Purpose

    For mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)

    Depositor

    Ayaz Najafov

    Insert

    AK1 (Ak1 Mouse)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV_puro_41589

    Plasmid

    #111632

    Purpose

    Complementation of Dicer_KO mESCs with mutated human DICER isoform

    Depositor

    Constance Ciaudo

    Insert

    FLAG-hsDicer (K70A) (DICER1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K70A

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p667-UBC-hcRed-V5_IDG-K

    Plasmid

    #135239

    Purpose

    Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    hcRed-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p663-UBC-V5-eGFP_IDG-K

    Plasmid

    #135265

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-CMV-Flag-hcRed_IDG-K

    Plasmid

    #135274

    Purpose

    Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-hcRed
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi-Pur-AktAR2-D

    Plasmid

    #125196

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    AktAR2-D
    

    Use

    Transposon

    Tags

    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPyCAG-Nanog(Q124A)-IP

    Plasmid

    #62993

    Purpose

    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog Q124A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Glutamine 124 (125 AA in Mouse Protein) t…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPyCAG-Nanog(M125A)-IP

    Plasmid

    #62994

    Purpose

    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog M125A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Methionine 125 (126 AA in Mouse Protein) …

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPyCAG-Nanog(Q138A)-IP

    Plasmid

    #62997

    Purpose

    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog Q138A Mutant (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    Changed Glutamine 138 (139 AA in Mouse Protein) t…

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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