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Viral Prep#73178-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.DepositorPromoterTagsNoneAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Ef1a-DIO EYFP (AAV1)
Viral Prep#27056-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsEYFP (Cre-dependent)Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDAC565
Plasmid#195242PurposeMammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)DepositorInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eCas9-GFP-U6-gRNA
Plasmid#79145PurposeAll-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cellsDepositorInserteSpCas9(1.1)
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
UseTagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
UseTags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cre Reporter
Plasmid#62732PurposeMammalian Cre recombinase reporterDepositorInsertLoxP-DsRED-STOP-LoxP-eGFP
UseCre/Lox and LentiviralTagsLoxP sitesExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight eGFP Hygro (w783-1)
Plasmid#26585Purpose2nd generation lentiviral vectorDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral; Tet-on advanced vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-hBMPR-1A CA
Plasmid#49527Purposeexpresses constitutively active human BMP receptor 1ADepositorInsertBMPR-1A CA (BMPR1A Human)
UseTagsExpressionMammalianMutationconstitutively active mutation (Q233D); also P2A …PromoterCMVAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only