168,238 results

1. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-LUC

   Plasmid

   #218630

   Purpose

   This plasmid contains luciferase under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LUC (LOC116160065 )
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. P4-PE

   Plasmid

   #211375

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   P4-PE
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX420

   Plasmid

   #229211

   Purpose

   Cytoplasmic-targeting Csm complex plasmid

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF-Con/Fon DREADD Gi-mCherry (AAV8)

   Viral Prep

   #177672-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon DREADD Gi-mCherry (#177672). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon DREADD Gi-mCherry plasmid DNA. nEF-driven, Cre- and Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   nEF

   Tags

   mCherry

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

7. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO.Sox9.Puro

   Plasmid

   #117269

   Purpose

   Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A and puromycin resistant cassette (C terminal …

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. phage UbiC G3BP1-GFP-GFP

   Plasmid

   #119950

   Purpose

   Lentiviral vector expressing G3BP1-GFP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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