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pMSCV-puro-PAX9

Plasmid

#75079

Purpose

retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pmiReport SHIP1 3' UTR

Plasmid

#26531

Depositor

David Baltimore

Article

O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

Insert

SHIP1 3'UTR (Inpp5d Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC2

Plasmid

#72711

Purpose

Lentiviral expression of shRNA against CRTC2 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC2

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 minimal promoter

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+N-eGFP_SAMD9L_R986C

Plasmid

#206176

Purpose

mammalian expression of SAMD9L R986C

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Tags

GFP

Expression

Mammalian

Mutation

R986C

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Ascl1

Plasmid

#74338

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagBcl10K31RK63R

Plasmid

#18719

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Lysine 31 to arginine and Lysine 63 to arginine.

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
hsa-miR-100 plix4.03

Plasmid

#121128

Purpose

inducible expression in cells

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-100 (MIR100 Human)

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY1026 COX8aN::miniSOG in pENTR[1-2]

Plasmid

#66752

Purpose

miniSOG fused to N terminal 29 aa of human Cox8a for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

COx8aN (N terminal 29 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGST-GstZ1

Plasmid

#163090

Purpose

Expressing mouse Gstz1 in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

GstZ1
GstZ1

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#2

Plasmid

#183120

Purpose

pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EPAS1-2

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-NIrD-Mefv iso1

Plasmid

#92108

Purpose

inducible expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pet16b ALIX Bro1 (1-359)

Plasmid

#80641

Purpose

Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1, residues 1-359 (PDCD6IP Human)

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-359

Promoter

T7

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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3578 pcDNA3 Bad S112A

Plasmid

#8797

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S112A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pRRL-VinculinVenus-S1033A

Plasmid

#211896

Purpose

Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

Insert

VinculinVenus-S1033A (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Mutation

mutated vinculin serine 1033 to alanine (S1033A),…

Promoter

CMV

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinVenus-S1033D

Plasmid

#211897

Purpose

Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

Insert

VinculinVenus-S1033D (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Mutation

mutated vinculin serine 1033 to aspartic acid (S1…

Promoter

CMV

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-hM4D(Gi)-mCherry

Plasmid

#190240

Purpose

Expresses iDREADD fused to mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4_OCT4_T234E_S235E

Plasmid

#40631

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Mutation

T234E_S235E

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPRIME-TREX-GFP-FF3

Plasmid

#11667

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV FLIP p53

Plasmid

#19745

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pCru5-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49226

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

Viral LTR (or CMV)

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AA)

Plasmid

#24240

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK8-ElonginB

Plasmid

#29504

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin B (ELOB Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pWPT-/CGA-mEGFP-IRES-mCherry

Plasmid

#49233

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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