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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,632 results for: chia

Showing: 3651 - 3700 of 5632 results

  1. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  2. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  3. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  4. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  5. pGEX-N-TEV-Twin-Strep

    Plasmid
    #222014
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli
    Depositor
    Chiaki Murakami
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222014-plasmid-map-sequence-id-457136

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  6. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  7. pHL 1915

    Plasmid
    #53022
    Purpose
    AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
    Depositor
    Han Lim
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and fimS fusion
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. PsbA2-HMGS-HMGR-ATOB-NPTI

    Plasmid
    #73338
    Purpose
    Construct expressing the upper MVA pathway in Synechocystis PCC 6803
    Depositor
    Anastasios Melis
    Insert
    HMGS-HMGR-ATOB-NPTI
    Use
    Tags
    Expression
    Mutation
    Promoter
    psbA2
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73338-plasmid-map-sequence-id-135049

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  23. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  24. pHL 636

    Plasmid
    #53015
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Inserts
    fimE
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator, RBS(st7), and T1 terminator
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    53015-plasmid-map-sequence-id-116679

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  30. Ec FkP-dnaN-pRSFDuet

    Plasmid
    #238342
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta(wt) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (wt) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    none (wt)
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238342-plasmid-map-sequence-id-472702

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  31. pAIP4_6IYE

    Plasmid
    #234237
    Purpose
    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234237-plasmid-map-sequence-id-464201

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  32. pAIP4_4IPC

    Plasmid
    #234229
    Purpose
    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4IPC (RPA1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234229-plasmid-map-sequence-id-464194

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  33. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  34. pAIP4_1KQX

    Plasmid
    #234181
    Purpose
    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KQX (rbp2a Zebrafish)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234181-plasmid-map-sequence-id-464146

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  35. pAIP4_4NFB

    Plasmid
    #234161
    Purpose
    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4NFB (PILRA Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234161-plasmid-map-sequence-id-464127

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  36. pAIP4_5EF9

    Plasmid
    #234154
    Purpose
    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234154-plasmid-map-sequence-id-464120

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  37. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

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  45. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  46. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3651 - 3700 of 5632 results