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pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

Plasmid

#124549

Purpose

Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

Insert

p65 promoter- p65 CDS-Clover

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ212 - pDESTR4-R3(cxTi10816, IV)

Plasmid

#34864

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pT-Rex Dest30 dn-cHSF1

Plasmid

#134736

Purpose

Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV/TO

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pdest EGFP Neo

Plasmid

#223052

Purpose

EGFP gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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726-Desmoglein2-myc

Plasmid

#32232

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmoglein2 (DSG2 Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-MCM7

Plasmid

#31212

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MCM7 (MCM7 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-FASCIN

Plasmid

#31207

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

FASCIN (FSCN1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-KD

Plasmid

#140313

Purpose

Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-Y1699C

Plasmid

#25048

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pdest METTL1-WT Neo

Plasmid

#223058

Purpose

METTL1 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-DEST-MatryoshCaMP6s

Plasmid

#100025

Purpose

Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

V5 Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-P110alpha

Plasmid

#40230

Depositor

Lynda Chin

Insert

P110 alpha (PIK3CA Bovine)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT1

Plasmid

#242131

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(WT)-mRuby2

Plasmid

#215448

Purpose

Piggybac expression vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Dest53-SHP-1 WT

Plasmid

#21791

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pDEST-DHFR F[3]-C (LEU2)

Plasmid

#177796

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[3]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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