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Plasmid#118204PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 EGFP-guide3
Plasmid#118168PurposeCRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDSDepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 CAMK1D TSS-guide2
Plasmid#118193PurposeCRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF
Plasmid#177267PurposeCRISPR vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB533-44B12m23-sfGFP
Plasmid#186778PurposeRNAP2 Ser5ph-mintbody expression vector for mammalian cellsDepositorInsert44B12m23-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-24xopto-TetO-no01
Plasmid#174887PurposePlasmid vector containing southern blot probe for opto-TetO in STREAMING-tagDepositorInsert24xopto-TetO
UseSouthern blot probeTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'GAC)
Plasmid#170129PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ec_bPAC
Plasmid#28135PurposeExpression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coliDepositorInsertE.coli adapted photoactivated adenylyl cyclase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA3b
Plasmid#47763Purposemammalian expression of rat PMCA3bDepositorInsertPMCA3b (Atp2b3 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV1997
Plasmid#37831DepositorInsertmCherry
UseTagsExpressionWormMutationPromoterAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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p3E LAP2b
Plasmid#105978Purpose3' element for Gateway cloning, lamina-associated polypeptide 2DepositorInsertLAP2b
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCD4 e/p Sal-(hCD2)
Plasmid#24070DepositorInsertCD2 (CD2 Human)
UseTagsmCD 4 promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW-eGFP-FHA
Plasmid#114129PurposeLentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatinDepositorInsertRNF8 FHA domain (amino acids 2-160) (RNF8 Human)
UseLentiviral; Doxycycline inducibleTagseGFPExpressionMammalianMutationaa 2-160PromoterTRE promoter, Tet ONAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
Plasmid#118155PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only