We narrowed to 15,817 results for: aga

1. CDK2 gRNA (BRDN0001148866)

   Plasmid

   #77191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK2 (CDK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-AR-R405A

   Plasmid

   #126052

   Purpose

   Expresses human androgen receptor (R405A) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R405A

   Promoter

   CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-ms-c-Myc-L420P

   Plasmid

   #50776

   Purpose

   retroviral expression of mouse c-Myc L420P mutant

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

   Insert

   c-Myc L420P (MYC, Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L420P (does not bind to Max)

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_intergenic-intergenic_1

   Plasmid

   #155077

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLCHKO_intergenic-intergenic_2

   Plasmid

   #155078

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSico_U6-PLOD2 sgRNA4

   Plasmid

   #136459

   Purpose

   Expression of gRNA against human PLOD2

   Depositor

   Antonio Amelio

   Article

   Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

   Insert

   gRNA against human PLOD2 (PLOD2 Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAMK2D gRNA (BRDN0001146878)

   Plasmid

   #77186

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CAMK2D

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CAMK2D (CAMK2D Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMG035 MBP-Axin∆BCD-His

   Plasmid

   #154053

   Purpose

   Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-AR-R405S

   Plasmid

   #126051

   Purpose

   Expresses human androgen receptor (R405S) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R405S

   Promoter

   CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LADL Bridge + Promoter Only Target

    Plasmid

    #127669

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCXIX_sh_hBcl10_#16

    Plasmid

    #75253

    Purpose

    retroviral shRNA vector against human BCL10, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against hBcl10 (BCL10 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQCXIX_sh_hBcl10_#2

    Plasmid

    #75254

    Purpose

    retroviral shRNA vector against human BCL10, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against hBcl10 (BCL10 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQCXIX_sh_hBcl10_#1

    Plasmid

    #75248

    Purpose

    retroviral shRNA vector against human BCL10, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against hBcl10 (BCL10 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mAC-POLR2A donor (Hygro)

    Plasmid

    #124496

    Purpose

    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SRC gRNA (BRDN0001146185)

    Plasmid

    #75651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCherry-imp α

    Plasmid

    #119719

    Purpose

    Expresses mCherry-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 251-529

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-Tet-On-PLXNB2-shRNA2

    Plasmid

    #98400

    Purpose

    Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-shDnd1-No7

    Plasmid

    #70068

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #7

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only