We narrowed to 2,618 results for: ubiquitin

1. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 Ub-NLRP1 S1213 P1214R

   Plasmid

   #166830

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 Ub-NLRP1 L1215

   Plasmid

   #166828

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221 Ub-NLRP1 S1213

   Plasmid

   #166827

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR221 Ub-NLRP1 L1218

   Plasmid

   #166829

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW_0005

   Plasmid

   #102837

   Purpose

   Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

   Depositor

   Brande Wulff

   Article

   Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

   Insert

   Sr22 PI573523 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDUET-GST AO7RE Y165A

   Plasmid

   #139428

   Purpose

   GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed tyrosine 165 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223 LC3B Q116P G120

    Plasmid

    #129291

    Purpose

    Gateway entry clone encoding human deconjugation-resistant LC3B

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-HA-RNF68 [dRING]

    Plasmid

    #126549

    Purpose

    Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RING domain deletion mutant [dRING mutation is a …

    Promoter

    LTR

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFL I3

    Plasmid

    #113514

    Purpose

    Expression of I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFL His-I3 V41A / W43A

    Plasmid

    #113515

    Purpose

    Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    V41A /W43A

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-6P-1 p37delta2-81

    Plasmid

    #113502

    Purpose

    Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 2-81

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p7054 pHAGE-P-CMVt-N-HA-GAW-COPE

    Plasmid

    #100144

    Purpose

    Expresses COPE

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    COPE (COPE Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6

    Plasmid

    #100140

    Purpose

    Encodes amino acids 4421-4834 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 4421-4834

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5

    Plasmid

    #100139

    Purpose

    Encodes amino acids 3550-4500 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 3550-4500

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4

    Plasmid

    #100138

    Purpose

    Encodes amino acids 2600-3600 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 2600-3600

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2

    Plasmid

    #100136

    Purpose

    Encodes amino acids 950-1750 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 950-1750

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge