We narrowed to 2,607 results for: ubiquitin

1. pGFP-mTet1s_K852E pc3915

   Plasmid

   #201705

   Purpose

   Expresses of GFP-tagged mTet1s_K852E in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852E (Tet1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to glutamate

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. rUBR5_2383-2442_pGEX6P1

   Plasmid

   #209791

   Purpose

   Bacterial expression of UBR5 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

   Insert

   Mlle domain of UBR5 (Ubr5 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2383-2442 only

   Promoter

   tac

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Duet1-6xHis-TEV-NDP52

   Plasmid

   #190194

   Purpose

   Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b p97-His D592Amb

   Plasmid

   #169022

   Purpose

   Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   D592TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221 Ub-NLRP1 S1213 P1214R

   Plasmid

   #166830

   Purpose

   pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Ubiquitin

   Expression

   Mutation

   P1214R

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW_0005

   Plasmid

   #102837

   Purpose

   Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

   Depositor

   Brande Wulff

   Article

   Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

   Insert

   Sr22 PI573523 (NEWENTRY Synthetic)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDUET-GST AO7RE Y165A

   Plasmid

   #139428

   Purpose

   GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed tyrosine 165 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETDUET-GST AO7RE I137A

    Plasmid

    #139319

    Purpose

    GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed isoleucine 137 to an alanine

    Promoter

    T7 promoter-1

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 LC3B Q116P G120

    Plasmid

    #129291

    Purpose

    Gateway entry clone encoding human deconjugation-resistant LC3B

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-HA-RNF68 [dRING]

    Plasmid

    #126549

    Purpose

    Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF68 (PCGF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RING domain deletion mutant [dRING mutation is a …

    Promoter

    LTR

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

    Plasmid

    #119318

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP III (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    C41A, C46A, C840A

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PHAGE-P CMVt N-HA hE6-AP I (G20V)

    Plasmid

    #119308

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (G20V) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G20V

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PHAGE-P CMVt N-HA hE6-AP I (C21Y)

    Plasmid

    #119304

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (C21Y) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    C21Y

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

    Plasmid

    #119313

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 55-376

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)

    Plasmid

    #119317

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP II (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-87

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)

    Plasmid

    #119310

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-64

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PHAGE-P CMVt N-HA hE6-AP I (R39H)

    Plasmid

    #119309

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (R39H) (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G20V

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

    Plasmid

    #119314

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 55-852

    Promoter

    CMV

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge