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pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Integrin beta1(1-455)-tev-BHCys

Plasmid

#27315

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
UBQ10:sXVE:(MCS)-S11

Plasmid

#108179

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z26

Plasmid

#120069

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P12

Plasmid

#120023

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-sport2-mGCN5 FU, FLAG Tagged

Plasmid

#23098

Depositor

Sharon Dent

Article

Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

Insert

mouse Gcn5 mRNA (Kat2a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

missing 50 bp upstream of ATG start site.

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
LentiGuidPuro-hTP63_sgRNA-1

Plasmid

#88854

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBI-eGFP-Rab22

Plasmid

#200264

Purpose

Expression of eGFP-Rab22 (human) in mammalian cells

Depositor

Guangpu Li

Article

Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

Insert

Rab22a (human cDNA) (RAB22A Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

Deleted myb domain (aa379-439)

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only