We narrowed to 1,472 results for: phr

1. pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174119

   Purpose

   Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203219

   Purpose

   Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-QNZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174118

   Purpose

   Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

   Plasmid

   #214885

   Purpose

   Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR

   Plasmid

   #67930

   Purpose

   Translational reporter - Emi1 UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs

   Plasmid

   #67929

   Purpose

   Translational reporter - control UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

   Plasmid

   #67931

   Purpose

   Translational reporter - ARHGAP5 UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188901

    Purpose

    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    SID-dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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