Addgene

We narrowed to 5,724 results for: ATC

Showing: 1481 - 1500 of 5724 results

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmSCN9A

Plasmid

#205636

Purpose

reRNA guide targeting Mus Musculus SCN9A

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmSCN9A (Scn9a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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USP7 shRNA-TRE

Plasmid

#225338

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

USP7 shRNA (Usp7 Mouse)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TRE (tetracycline-responsive element)

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnTS-E1015A

Plasmid

#213415

Purpose

Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1015A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1015 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnTS-E1021A

Plasmid

#213416

Purpose

Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1021 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnCS-E1015A

Plasmid

#213412

Purpose

Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnCS-E1015A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1015 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VcnCS-E1021A

Plasmid

#213413

Purpose

Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnCS-E1021A (VCL Chicken, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Mutated vinculin glutamic acid 1021 to alanine (E…

Promoter

CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-ST-P2A-pSC-CAAX

Plasmid

#207637

Purpose

A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX

Use

Tags

Expression

Mammalian

Mutation

Amber stop codon at SC's critical lysine

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 2

Plasmid

#193592

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 2 (RELA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpb_v2

Plasmid

#177252

Purpose

Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpd_v2

Plasmid

#177254

Purpose

Expresses Cebpa_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpd_v2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpa_v2 (opti)

Plasmid

#177246

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Use

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Mutation

Promoter

ptac

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ctcf-R

Plasmid

#122320

Purpose

Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ctcf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

Plasmid

#120911

Purpose

Expresses BFP tagged Ptch1-B (V111FL114FI766F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

Plasmid

#120908

Purpose

Expresses Ptch1-B (V111FL114FW115A)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

Plasmid

#120907

Purpose

Expresses Ptch1-B (I766FV111F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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