We narrowed to 18,347 results for: Uty

1. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

   Plasmid

   #218462

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr1273Asp substitution

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1A Prk-

   Plasmid

   #162694

   Purpose

   p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Prk inactive (K20M S21A native protein, this map …

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUG-T2A-F241S

   Plasmid

   #140740

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENF241S (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   F241S

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUG-T2A-W274L

   Plasmid

   #140742

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTENW274L (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   W274L

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUG-T2A-D252G

   Plasmid

   #140741

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTEND252G (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   D252G

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183606

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-LUC2CP/ARE

   Plasmid

   #62857

   Purpose

   Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   destabilizing sequences (DS) added to the C termi…

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVAX1-BMP2

   Plasmid

   #137909

   Purpose

   Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Insert

   BMP2 (BMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH624-ssr

   Plasmid

   #199241

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   single-stranded DNA recombineering
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptrc

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

    Plasmid

    #135465

    Purpose

    Membrane-anchored TF used for yeast directed evolution of TEV protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
    

    Use

    Tags

    BFP, flag

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

    Plasmid

    #233549

    Purpose

    A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIA4.721-Pansio

    Plasmid

    #214441

    Purpose

    BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RSV-Flag-Brd2

    Plasmid

    #86614

    Purpose

    Flag tagged mouse Brd2 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd2 (Brd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCB’

    Plasmid

    #162703

    Purpose

    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5513T>G (tetR E37A); 7758G>T (second tet op…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMIA4.721-Keppel

    Plasmid

    #214444

    Purpose

    BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMIA4.721-Olônne

    Plasmid

    #214443

    Purpose

    BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RSV-Flag-Brd4

    Plasmid

    #86616

    Purpose

    Flag tagged mouse Brd4 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd4 (Brd4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJBEI-6411

    Plasmid

    #47050

    Purpose

    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge