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Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
6MIW
Plasmid#127291PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertHUWE1 (HUWE1 Human)
UseTags6xHis-TEV (MHHHHHHSSGRENLYFQG)ExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRIP12-V2_A755-A841
Plasmid#194759PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTRIP12 (TRIP12 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK635
Plasmid#72473PurposepET28a modified to contain TEV protease fusion tag instead of T7 tagDepositorTypeEmpty backboneUseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM75
Plasmid#224249PurposepET28a MWG-ubiquitin-G77-C78DepositorInsertMWG-ubiquitin-G77-C78
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6-mNeon
Plasmid#178460PurposeThe mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a mScarlet
Plasmid#178543PurposeThe mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmScarlet
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pH6-2xmNeon
Plasmid#178462PurposeA tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.DepositorInsertmNeonGreen dimer
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTorD-H6
Plasmid#169039PurposeEscherichia coli TorD with C-terminal 6xHis in pET28a.DepositorInsertTorD-H6
UseTags6xHisExpressionBacterialMutationThe mutation N at position 46 was changed back to…PromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW016
Plasmid#131820PurposepET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6-spTorA-mCherry
Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
UseTags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6-mNeonC
Plasmid#178461PurposeThe mNeonGreen fluorescent protein with an N-terminal 6xHis and a C-terminal free-Cysteine in pET28aDepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationFree cysteine at the C-terminusPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6-2xmNeonC
Plasmid#178463PurposeA tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.DepositorInsertmNeonGreen dimer
UseTags6xHisExpressionBacterialMutationFree cysteine at the C-terminus of the dimerPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z3-Nluc
Plasmid#174486PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ3-Nluc
UseTags6x Histidine tagExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
UseTags6x Histidine tag (N terminal on insert)ExpressionBacterialMutationPromoterT7lac promoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only