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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X (G124L, I127A)

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-DN-REST

Plasmid

#61533

Purpose

Expresses DN-REST in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

REST (REST Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P73, Dominant negative REST (contains the Zf DNA-…

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-HDAC4 3SA

Plasmid

#45637

Depositor

Tso-Pang Yao

Article

Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

Insert

HDAC4 (HDAC4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

3SA

Promoter

CMV

Available Since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

Plasmid

#161993

Purpose

Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

R286A Y287A K288A

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIH-tag RFPt- Rab11a WT

Plasmid

#161992

Purpose

Retroviral expression vector containing Rab11a tagged with RFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

RAB11A (RAB11A Canis Lupus)

Use

Retroviral

Tags

RFP

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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