168,238 results

1. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV5)

   Viral Prep

   #100845-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   Syn

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pHREAC

   Plasmid

   #134908

   Purpose

   Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   double 35S

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-Soma-jGCaMP8f (AAV1)

   Viral Prep

   #169258-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8f (#169258). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8f plasmid DNA. Synapsin-driven neuronal expression of soma-targeted calcium sensor jGCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

4. mEGFP-RhoA-C1

   Plasmid

   #29674

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

   Insert

   RhoA (RHOA Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALIX-mNeonGreen

   Plasmid

   #191191

   Purpose

   Full-length ALIX with C-terminal mNeonGreen tag.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (PDCD6IP Human, Synthetic)
   

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL1765 (pSPIN-R, R*MmeI)

   Plasmid

   #160734

   Purpose

   Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. attP-puro donor

   Plasmid

   #182139

   Purpose

   attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CNR1-Tango

   Plasmid

   #66254

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CNR1 (CNR1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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