Addgene

We narrowed to 12,329 results for: BASE;

Showing: 1501 - 1520 of 12329 results

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S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 V82G ABEMax Strategy 3

Plasmid

#135363

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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PB-BE3-NLS

Plasmid

#119030

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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hCtRNA_VT

Plasmid

#186716

Purpose

Vector template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

hCtRNA and gRNA scaffold

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

Plasmid

#158158

Purpose

Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-miniTurbo-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTNS1

Plasmid

#64967

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p370_DG

Plasmid

#234693

Purpose

Golden gate domesticated pET28b with 3C-mNeongreen-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-mNeongreen-His10

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p277_DG

Plasmid

#234695

Purpose

Golden gate domesticated pET28b for scarless cloning with His6-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p357_DG

Plasmid

#234700

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p349_DG

Plasmid

#234679

Purpose

Golden gate domesticated pET28b with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p351_DG

Plasmid

#234681

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p364_DG

Plasmid

#234686

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Opsin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8 and Opsin

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p358_DG

Plasmid

#234701

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p359_DG

Plasmid

#234702

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p348_DG

Plasmid

#234678

Purpose

Golden gate domesticated pET28b with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge