We narrowed to 7,486 results for: CIT

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pET28a-R4-uAb

Plasmid

#101801

Purpose

Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL

Plasmid

#163574

Purpose

express wild type Dcp2 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Expression

Mammalian

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_human_spike-in

Plasmid

#125152

Purpose

Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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AP-PTEN(med)

Plasmid

#168005

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T7PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S345A

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pReporter_2

Plasmid

#60563

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_2

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST1K18ms

Plasmid

#72646

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLenLox_U6 Nlgn3

Plasmid

#59359

Purpose

Lentiviral expression vector: Neuroligin 3 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 3 shRNA (rat) (Nlgn3 Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pReporter_7

Plasmid

#60567

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_7

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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CMV500 A-SREBP2

Plasmid

#33358

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Insert

SREBP2

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-T1

Plasmid

#190825

Purpose

For mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

T1 (an RNA aptamer of O-GlcNAc Transferase)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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