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Plasmid#225486PurposeTet-on lentiviral vector for controlled expression of secretable and permeable EGFPDepositorInsertIgκ leader and TAT peptide
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)GPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARP3-V5-TurboID
Plasmid#195144Purposemeasure COR1C interaction boichemicallyDepositorInsertACTR3 (ACTR3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-10
Plasmid#140143PurposeEvaluating the impact of high sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoternoneAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-.008
Plasmid#140141PurposeEvaluating the impact of low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly_spike-in
Plasmid#125151PurposeVector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cellsDepositorInsertD. pseudoobscura peak #47 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 4
Plasmid#99319PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
UseTagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA3mt
Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII-ShadowG
Plasmid#167475PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
UseTagspHluorinExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only