Skip to main content
Addgene
Showing: 1501 - 1520 of 2388 results
  1. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid
    #207151
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Insert
    GNAO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid
    #207152
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Insert
    GNAO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pYFP-x-10

    Plasmid
    #140143
    Purpose
    Evaluating the impact of high sensor concentration on information transmission of a genetic sensor.
    Depositor
    Insert
    lacY (lacY )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D37, I38, and N39 are changed to nonsense (stop) …
    Promoter
    none
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pYFP-x-.008

    Plasmid
    #140141
    Purpose
    Evaluating the impact of low sensor concentration on information transmission of a genetic sensor.
    Depositor
    Insert
    lacY (lacY )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D37, I38, and N39 are changed to nonsense (stop) …
    Promoter
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. mCit-4aa-SNAP25B

    Plasmid
    #53235
    Purpose
    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
    Depositor
    Insert
    SNAP-25B (Snap25 Rat)
    Use
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1501 - 1520 of 2388 results