We narrowed to 42,232 results for: Tro;

1. Human 3' HP1a AID GFP PuroR

   Plasmid

   #127906

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   HP1 (CBX5 Mustard Weed, Human)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Hp1a

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-td-mCherry-Cdk5rap2-CTD

   Plasmid

   #196854

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-AcGFP-Cdk5rap2-CTD

   Plasmid

   #196853

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINSPECT:IL2_SigP:NLuc

   Plasmid

   #197266

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   Interleukin-2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINSPECT:NEAT1-v2_SigP:NLuc

   Plasmid

   #197268

   Purpose

   Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Insert

   NEAT1-v2 homology arms
   

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR D-TOPO-C3_9

   Plasmid

   #60258

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   NKX6-1 enhancer (NKX6-1 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129048

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA8 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

   Plasmid

   #129041

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA1 (huTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR D-TOPO-C3_10

   Plasmid

   #60259

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   RFX6 enhancer (RFX6 Human)
   

   Use

   Entry vector

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR D-TOPO-C3_25

    Plasmid

    #60268

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ROCK1 enhancer (ROCK1 Human)
    

    Use

    Entry vector

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSG5LHARB1sgresistant

    Plasmid

    #229856

    Purpose

    RB1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Inserts

    RB1 (RB1 Human)
    Rb1 (RB1 Human)
    

    Tags

    HA (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    changed a bp24 to g; g bp27 to c, and c bp30 to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. sh2g-ZMYND8-human

    Plasmid

    #201406

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. sh4p-ZMYND8-mouse

    Plasmid

    #201405

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid

    #197267

    Purpose

    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Insert

    NEAT1-total homology arms
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196855

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

    Plasmid

    #163341

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid

    #129047

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA7 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid

    #129049

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA9 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid

    #129050

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid

    #129051

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA11 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge