161,398 results

1. pCMVHA hEZH2

   Plasmid

   #24230

   Purpose

   Mammalian expression of human EZH2 with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG2

   Plasmid

   #104174

   Purpose

   sgRNA expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-RP

   Plasmid

   #60513

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-VN155(I152L)

   Plasmid

   #27097

   Depositor

   Chang-Deng Hu

   Article

   Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

   Insert

   VN155(I152L)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   I152L

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA2c-RFP

   Plasmid

   #35326

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CFP-Parkin

   Plasmid

   #47560

   Purpose

   CFP tagged Parkin for colocalization study

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229770

   Purpose

   Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

   Plasmid

   #229769

   Purpose

   Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
   

   Use

   Luciferase

   Tags

   SmBiT

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-PASS

    Plasmid

    #193970

    Purpose

    Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

    Depositor

    Guangwei Du

    Article

    Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

    Insert

    PA biosenor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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