We narrowed to 2,424 results for: MEA;

1. mKOkappa-2A-mTurquoise2

   Plasmid

   #98837

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKOkappa-2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PIP2-YFP(ST) sensor

   Plasmid

   #170354

   Purpose

   YFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

   Depositor

   Kees Jalink

   Article

   van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

   Insert

   YFP(ST)-PH (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mKO2-2A-mTurquoise2

   Plasmid

   #98838

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKO2-2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PIP2-CFP(ST) sensor

   Plasmid

   #170355

   Purpose

   CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

   Depositor

   Kees Jalink

   Article

   van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

   Insert

   CFP(ST)-PH (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-51b(+)_NADP-Snifit

   Plasmid

   #113913

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IP-GFP-LC3-mRuby3

   Plasmid

   #184898

   Purpose

   Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   LC3B
   

   Use

   Tags

   EGFP and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGAN213

   Plasmid

   #129270

   Purpose

   Control for measuring background levels of yTRAP reporter protein

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   min pCYC1

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT2P-EF-Q7mu

   Plasmid

   #129303

   Purpose

   Positive control for ATP measurement, mitochondria outer membrane, Tol2 transposon

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

   Insert

   QUEEN-7mu
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYFP-lacY-.008

    Plasmid

    #140140

    Purpose

    Evaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSTAP-seq_fly-ctrl

    Plasmid

    #86380

    Purpose

    Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Type

    Empty backbone

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDLterm

    Plasmid

    #211903

    Purpose

    Base plasmid for dual-luciferase constructs to measure terminator strength

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S enhancer + 35S minimal promoter

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid

    #207151

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)G

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid

    #207152

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)G

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARP3-V5-TurboID

    Plasmid

    #195144

    Purpose

    measure COR1C interaction boichemically

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYFP-x-10

    Plasmid

    #140143

    Purpose

    Evaluating the impact of high sensor concentration on information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D37, I38, and N39 are changed to nonsense (stop) …

    Promoter

    none

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYFP-x-.008

    Plasmid

    #140141

    Purpose

    Evaluating the impact of low sensor concentration on information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D37, I38, and N39 are changed to nonsense (stop) …

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSTAP-seq_fly_spike-in

    Plasmid

    #125151

    Purpose

    Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    D. pseudoobscura peak #47 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge