Purpose
pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
Inserts
human codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (
ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
Mutation
E488Q, only the deaminase domain (aa 276-702 are …
Promoter
EFS (short EF1alpha) and hU6
Available Since
Oct. 14, 2021
Availability
Academic Institutions and Nonprofits only