We narrowed to 12,265 results for: cel.2

1. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

   Plasmid

   #201677

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette

   Depositor

   Timo Otonkoski

   Article

   Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

   Insert

   MIR302-3g-EEA-2g-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pExp-His-zBasic-RBD-Avi

   Plasmid

   #195000

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Tags

   Avi-tag and His8-Zbasic

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1 (+) FLAG-Ago2 S387E

   Plasmid

   #60653

   Purpose

   Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   CMV-FLAG-Ago2 (N-term tag)

   Expression

   Mammalian

   Mutation

   S387E mutant

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA 3.1 (+) FLAG-Ago2 S387A

   Plasmid

   #60652

   Purpose

   Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   CMV-FLAG-Ago2 (N-term tag)

   Mutation

   S387A mutant

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST26 Ago2 S387A

   Plasmid

   #59905

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   S387A, phospho-null alanine

   Promoter

   CMV

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST26 Ago2 5A

   Plasmid

   #60651

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   mutated five serines to alanines

   Promoter

   CMV

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST26 Ago2 4A

   Plasmid

   #59907

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   mutated four serines to alanines

   Promoter

   CMV

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CamKIIa-ChR2-EGFP

   Plasmid

   #114216

   Purpose

   Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Channelrhodopsin 2
   

   Use

   AAV and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CamKIIa 1.3 kb

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pk335-BFP

   Plasmid

   #227505

   Purpose

   Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (AAV8)

    Viral Prep

    #183519-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (#183519). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE plasmid DNA. CaMKIIa-driven expression of ChRmine-oScarlet-KV 2.1. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Promoter

    CaMKII

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

11. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

    Plasmid

    #40640

    Depositor

    Brenda Lilly

    Article

    Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCer-TG2-YFP

    Plasmid

    #85471

    Purpose

    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

    Depositor

    Jeffrey Keillor, Ray Truant

    Article

    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

    Insert

    hTG2 (TGM2 Human)
    

    Tags

    EYFP and mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

    Plasmid

    #131355

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

    Plasmid

    #131354

    Purpose

    Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Caspase-1 (C285A)

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Venus-Bad-2A-pEGFP-C1

    Plasmid

    #166748

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bad-2A (BAD Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. anxA9-GFP

    Plasmid

    #107198

    Purpose

    Expresses human annexin A9-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

    Insert

    annexin A9 (ANXA9 Human)
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTF

    Plasmid

    #162784

    Purpose

    Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Type

    Empty backbone

    Tags

    10xHis and FLAG

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Syntaxin4-myc-myc-His

    Plasmid

    #12377

    Depositor

    Thomas Weimbs

    Article

    Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

    Insert

    Syntaxin4 (STX4 Human)
    

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Cloned in modified PCDNA4 vector (double myc)

    Promoter

    CMV⁄TO

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL Neo Myr Flag SPHK2

    Plasmid

    #20571

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pEY40

    Plasmid

    #191044

    Purpose

    ceh-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    ceh-2 (ceh-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge