We narrowed to 81,810 results for: Mycs

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pCas9-EcRT-GAL-Kan

Plasmid

#232092

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Human, Synthetic, Budding Yeast)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-T1977R

Plasmid

#69011

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-T1977R (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Threonine 1977 to Arginine

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pYL236

Plasmid

#173184

Purpose

CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCR2.1-promoter-mIL-6

Plasmid

#109296

Purpose

IL-6 promoter reporter construct

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Mutation

CMV promoter in pEGFP-N1 replaced with murine IL-…

Promoter

murine IL-6 promoter

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Tags

His6

Expression

Yeast

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

Plasmid

#123346

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-CKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only