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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,376 results for: 683

Showing: 1541 - 1560 of 2376 results

  1. LBR(R583Q)-FKBP-GFP

    Plasmid
    #222423
    Purpose
    To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(R583Q) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    R583Q
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222423-plasmid-map-sequence-id-439219

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  2. LBR(N547D)-FKBP-GFP

    Plasmid
    #222422
    Purpose
    To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR(N547D) (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    N547D
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222422-plasmid-map-sequence-id-439218

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  3. Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

    Plasmid
    #216403
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Alanine 51, 56 and Glutamine 70, 443 to P…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216403-plasmid-map-sequence-id-430104

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  4. Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

    Plasmid
    #216404
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Alanine 51, 56, Glutamine 70, 443 and Asp…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216404-plasmid-map-sequence-id-430105

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  7. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  8. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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Showing: 1541 - 1560 of 2376 results