We narrowed to 18,609 results for: Met

1. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF712-pET28a-Hs-delta44-GCDH-NHis wt

   Plasmid

   #85113

   Purpose

   expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta44) GCDH (GCDH Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   silent SNP: G1041T

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX304-ARF5-mNeonGreen

   Plasmid

   #162025

   Purpose

   Expression of tagged ARF WT

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF5 (ARF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174647

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   CMV (with TetOn)

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC010.AAV

   Plasmid

   #171175

   Purpose

   Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   BBa_J23119 and Sp.pCas9, BBa_J23107

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3b(1454/3172)P2P-mut

   Plasmid

   #21405

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   hypoxia response element mutated

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pdest N-FLAG EGFP puro

   Plasmid

   #223056

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdest WDR4 Puro

   Plasmid

   #223062

   Purpose

   WDR4 gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   WDR4 (WDR4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. rag2:RAB3IP_CE_pISceI

   Plasmid

   #223044

   Purpose

   Express RAB3IP gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   RAB3IP (RAB3IP Human)
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. rag2:WDR4_CE_pISceI

    Plasmid

    #223051

    Purpose

    Express WDR4 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    WDR4 (WDR4 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. rag2:TSFM_CE_pISceI

    Plasmid

    #223031

    Purpose

    Express TSFM gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    TSFM (TSFM Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. rag2:OS9_CE_pISceI

    Plasmid

    #223023

    Purpose

    Express OS9 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    OS9 (OS9 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. rag2:TSPAN31_CE_pISceI

    Plasmid

    #223025

    Purpose

    Express TSPAN31 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    TSPAN31 (TSPAN31 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-20nmEML-mRFP

    Plasmid

    #231178

    Purpose

    Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    20nmEML-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-30nmEML-mRFP

    Plasmid

    #231179

    Purpose

    Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    30nmEML-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-15nmEML-mRFP

    Plasmid

    #231177

    Purpose

    Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    15nmEML-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid

    #226247

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACB120

    Plasmid

    #222213

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(K10N, S11R, W15C, G76V, G258D)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Contains five amino acid mutations: K10N, S11R, W…

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKG-GW2-NgBR R290H

    Plasmid

    #203167

    Purpose

    Yeast expression vector for hNUS1 R290H

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R290H

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSF-G1TMSNKRS

    Plasmid

    #198323

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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