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We narrowed to 12,129 results for: NUC

Showing: 1541 - 1560 of 12129 results

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pcDNA4/TO/FLAG-hPMR1-Tev-Bio

Plasmid

#80125

Purpose

Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Article

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Use

Tags

FLAG and biotinylation

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-1.03kb-EGFP

Plasmid

#153164

Purpose

Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pK-SAN1-Flag

Plasmid

#117163

Purpose

Expresses Flag-tagged SAN1 nuclease in mammalian cells.

Depositor

Ian Macara

Article

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

SAN1 (FAM120B Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

Plasmid

#121834

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-Cas9(3.7) (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Tmem38A mRFP-N2 (800)

Plasmid

#62007

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem38A (TMEM38A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pOPTXcGMPRELUC

Plasmid

#68503

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)

Use

Tags

luciferase

Expression

Bacterial and Plant

Mutation

3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

Promoter

OPTX promoter with cGMPRE

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

Plasmid

#121821

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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WFS1 mRFP-N2 (891)

Plasmid

#62018

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
TAPBPL pEGFP-C3 (1765)

Plasmid

#62055

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

TAPBPL (TAPBPL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pNIA-CEN-Venus-FLAG-LINXa4-Bre1

Plasmid

#73618

Purpose

Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

Venus

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

Plasmid

#121828

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

x-dCas9(3.7) (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-delta-N

Plasmid

#27419

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
NET4 pEGFP-N2 (1201)

Plasmid

#62041

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

Net4 (TMEM53 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
r-22. pT7-7-SNCA-9Asn

Plasmid

#107297

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-9Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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t2. pT7-7-SNCA-5Lys

Plasmid

#107294

Purpose

Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Lys (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r-6. pT7-7-SNCA-2Asn

Plasmid

#107296

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-2Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOPTXGARE

Plasmid

#68542

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with GARE (AT1G33440 Mustard Weed)

Use

Tags

luciferase

Expression

Bacterial and Plant

Mutation

5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

Promoter

OPTX promoter with GARE

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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