We narrowed to 17,361 results for: She

1. piGECI-N1

   Plasmid

   #160421

   Purpose

   Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBITE-EGFRvIII-F265-OKT3

   Plasmid

   #190679

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRc/CMV2 Dvl-2-Myc

   Plasmid

   #42194

   Depositor

   Robert Lefkowitz, Shin-ichi Yanagawa

   Article

   Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

   Insert

   Dvl 2 (Dvl2 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pJL1-sfGFP-AQNAT

   Plasmid

   #198211

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_AQNAT
   

   Use

   Tags

   6x His Tag and AQNAT mutated (non-permissible) gl…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1-sfGFP-MOOR

   Plasmid

   #198212

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_MOOR
   

   Use

   Tags

   6x His Tag and MOOR glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TfR-pHoran4

   Plasmid

   #61547

   Purpose

   Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

   Depositor

   David Perrais

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   Transferrin receptor (TFRC Human)
   

   Use

   Tags

   pHoran4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXJ-His-VTCN1

   Plasmid

   #232664

   Purpose

   transient expresses VTCN1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   VTCN1 (VTCN1 Human)
   

   Use

   Tags

   His and His (retained after signal peptide cleava…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac HT JS-Munc18c

    Plasmid

    #135555

    Purpose

    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18c
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-24p3/LCN2-Luc

    Plasmid

    #25463

    Depositor

    Sarah Gaffen

    Article

    Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

    Insert

    24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    June 25, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pXJ-Myc-GATA6

    Plasmid

    #232648

    Purpose

    transient expresses GATA6 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ-Myc-GATA4

    Plasmid

    #232647

    Purpose

    transient expresses GATA4 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST1374-N-NLS-flag-linker-Cas9-H840A

    Plasmid

    #51129

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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