We narrowed to 3,315 results for: cgas

1. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

   Plasmid

   #129708

   Purpose

   Express cre-dependent KASH-EGFP and scramble shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA-his-HYB

   Plasmid

   #64333

   Purpose

   his3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of his3 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTC394

   Plasmid

   #91224

   Purpose

   protoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_(GLuc)_INT[BoBS]

   Plasmid

   #68431

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. shCDK4/6(1)

   Plasmid

   #73554

   Purpose

   vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pRRL-mU6-GFPGO2-IRES-mScarletI

   Plasmid

   #136896

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   2xNLS on GFP, 3xNLS on mScarlet

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p CIneo-RL-Let7-3xBulgeB-mut

   Plasmid

   #115369

   Purpose

   Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

   Depositor

   Ramesh Pillai, Witold Filipowicz

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6_(GLuc)_INT[3xPP7_SL]

   Plasmid

   #68424

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. BII-Sh-FUCCI

   Plasmid

   #133363

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACM4-ACA

    Plasmid

    #49809

    Purpose

    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent site-directed mutagenesis to remove SalI s…

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ23119-sgRNA

    Plasmid

    #113654

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter J23119 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-UFM1 sgRNA2

    Plasmid

    #134634

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA2 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMP-G9A_1

    Plasmid

    #36395

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pPBO.ACT005

    Plasmid

    #182715

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1104

    Plasmid

    #119248

    Purpose

    knockdown pqsC in P. aeruginosa

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA pqsC (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-STAG2 shRNA 1221

    Plasmid

    #31978

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pSIN-Skil-gRNA2

    Plasmid

    #180371

    Purpose

    targeting mouse Skil/SnoN gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Skil targeting gRNA (Skil Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. plko.1/shMCT4 #474

    Plasmid

    #99597

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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