We narrowed to 6,952 results for: crispr cas9 plasmids

1. pJB172

   Plasmid

   #86992

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Insert

   gRNA targeting pil1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330_SMAD exon 2 gRNA

   Plasmid

   #68350

   Purpose

   px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   SMAD exon 2 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330_P53 exon 8 gRNA

   Plasmid

   #68349

   Purpose

   px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 8 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330_P53 exon 7 gRNA

   Plasmid

   #68351

   Purpose

   px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 7 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330_PTEN exon 5 gRNA

   Plasmid

   #68348

   Purpose

   px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   PTEN exon 5 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAT39

   Plasmid

   #204289

   Purpose

   For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-EGFP+H3:H26
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT10

   Plasmid

   #204293

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-dSa VP64 Afp

   Plasmid

   #99697

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH2972

   Plasmid

   #100956

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH2971

    Plasmid

    #100955

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

    Depositor

    James Haber

    Article

    Ranjith et al Protocol Exchange (2017)

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH2970

    Plasmid

    #100954

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker

    Depositor

    James Haber

    Article

    Ranjith et al Protocol Exchange (2017)

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT32

    Plasmid

    #204297

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAT77

    Plasmid

    #204295

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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