We narrowed to 18,708 results for: met

1. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdest EGFP Neo

   Plasmid

   #223052

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAd-Track Flag HA PGC-1 alpha

   Plasmid

   #14426

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Adenoviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Last four amino acids of PGC-1a removed to make t…

   Promoter

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLV hUbC-dCas9-dMQ1-EGFG

   Plasmid

   #89794

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   hUbC

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ITR-PytR(A2.1)-mCherry

   Plasmid

   #204869

   Purpose

   Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA mutant "A2.1"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4G, T65C, T66C, T67T

   Promoter

   U6

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 EWS-myc-HIS

   Plasmid

   #46386

   Purpose

   expresses myc, HIS tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

   Plasmid

   #85111

   Purpose

   expression of recombinant human mature (delta19) ETFalpha wt without tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta19) ETFalpha (ETFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUltraI-Tet3.0[TAA]

   Plasmid

   #164580

   Purpose

   Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mb Tet3.0RS (R2-3)
   Mm tRNA[TAA]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L170G, N311G, C313A

   Promoter

   ProK and TacI

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

   Plasmid

   #71216

   Purpose

   dCas9 fused to human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A catalytic domain
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAd-Track HA PGC-1 alpha

    Plasmid

    #14427

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Promoter

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

    Plasmid

    #71218

    Purpose

    dCas9 fused to the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A(E752A)
    

    Use

    Lentiviral

    Tags

    SPdCas9-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX304-ARF5-mNeonGreen

    Plasmid

    #162025

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174647

    Purpose

    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    CMV (with TetOn)

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPPC010.AAV

    Plasmid

    #171175

    Purpose

    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    BBa_J23119 and Sp.pCas9, BBa_J23107

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3b(1454/3172)P2P-mut

    Plasmid

    #21405

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    hypoxia response element mutated

    Promoter

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. rag2:RAB3IP_CE_pISceI

    Plasmid

    #223044

    Purpose

    Express RAB3IP gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    RAB3IP (RAB3IP Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. rag2:WDR4_CE_pISceI

    Plasmid

    #223051

    Purpose

    Express WDR4 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    WDR4 (WDR4 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rag2:TSFM_CE_pISceI

    Plasmid

    #223031

    Purpose

    Express TSFM gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    TSFM (TSFM Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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