We narrowed to 6,252 results for: nls

1. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

   Plasmid

   #133788

   Purpose

   CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized St3Cas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmyc-GFP-TNRC6A-NLS/NES-mut

   Plasmid

   #42002

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

   Promoter

   CMV

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

   Plasmid

   #133790

   Purpose

   CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized NmeCas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-eGFP-NLS-RNF168 delta RING

   Plasmid

   #133983

   Purpose

   mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of RING; siRNA resistant sequence: GAGGA…

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79889

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-xFNLS-T2A-EGFP-ires-puro

   Plasmid

   #121171

   Purpose

   CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   xFNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-myc NES-mCE NLS-Flag

   Plasmid

   #82468

   Purpose

   Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   Myc-NES-mCE-Flag (Rngtt Mouse, HIV)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

   Plasmid

   #68279

   Purpose

   SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Far1DS NLS mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   RPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CMV-NLS-mTurquoise2-optoNES-WPRE

    Plasmid

    #159962

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2-optoNES
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

    Plasmid

    #68278

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCitrine
    

    Tags

    mCitrine

    Expression

    Yeast

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

    Plasmid

    #27425

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of NLS-Nuclear Localization Signal: Nucl…

    Available Since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    Aedes aegypti poly-ubiquitin

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

    Plasmid

    #68280

    Purpose

    SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Mkk2DS NLS mCherry (MKK2 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    RPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

    Plasmid

    #68274

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS

    Plasmid

    #241912

    Purpose

    Zebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

    Depositor

    Balázs Enyedi

    Article

    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

    Insert

    QUAS enhancer driving mNeonGreen-NES and mKate2-NLS
    

    Use

    Zebrafish tol2 transgenesis

    Promoter

    QUAS

    Available Since

    Oct. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVss-G1B3-nlsCre-WPRE-miR124T-SYNpolyA

    Plasmid

    #245080

    Purpose

    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Insert

    nls-Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SIN-cPPT-G1B3-nlsCre-WPRE-miR124T

    Plasmid

    #245072

    Purpose

    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Insert

    nls-CRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL6puro-JeT-pmRevErbA-miRFP713-NLS-Pest

    Plasmid

    #240110

    Purpose

    A lentiviral (infrared) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+miRFP713-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added JeT Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

    Plasmid

    #241843

    Purpose

    Expresses an actuator of Opto-p53 in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

    Insert

    VP16
    

    Tags

    CRY2 and miRFP703

    Expression

    Mammalian

    Mutation

    VP16 minimal activation domain x 3

    Promoter

    CAGGS promoter

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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